Blood is transported from the unit car (MU) to the Blood Donor Unit (UDD) of the Indonesian Red Cross (PMI) using a conventional cooler box without using tools that maintain temperature stability and maintain the effect of vibration. Such conditions are very likely to decrease the quality of blood during the transportation process. If at the time of transportation from MU to UDD PMI, a lot of blood damage is found, it will cause losses both to the donor, PMI and during blood transfusion activities. Blood and various blood components can be transfused separately according to needs. Blood is composed of various components, namely erythrocytes (red blood cells), concentrated platelets (thrombocyte concentrate), cryopresspitate, and frozen fresh plasma (fresh frozen plasma). The storage of blood products and their components according to the optimal temperature range during transportation largely determines the survival of blood cells and reduces the faster growth of bacteria contained in the blood bag, so the blood transport process must meet the requirements that have been set. Transportation in one bag of whole blood to ensure good blood quality is maintained in a temperature of 2-6ºC. Maintaining blood quality requires temperature stability during the transportation process. In addition to maintaining temperature stability to maintain blood quality, it is also necessary to maintain vibrational stability during transportation. Based on the above problems, the purpose of this study is to analyze the effect of temperature in the box cooler toward blood quality during the transportation process.