The Saudi vision 2030 is aiming to foster research and development through its development and innovation programs. The University of Tabuk recognizes the significance of scientific research and its integral role in the development of the Community and improvement of patients' care. Therefore, this research aimed to assess the barriers to and perception of research among staff members, Faculty of Medicine, University of Tabuk. A cross-sectional study was conducted among 94 faculty members, Faculty of Medicine, University of Tabuk, Saudi Arabia (response rate 40%). The study was conducted in September and October 2021. A web-based questionnaire consists of two parts. The first collected demographic data, and the second inquiries about the type of research published. The reasons behind conducting research, and challenges or barriers to research (support, knowledge were reported. In addition, other barriers include time constraints, social factors, and lack of access to statistical software like SPSS. The Statistical Package for Social Sciences was used for data analysis. There were 42 staff members from different Departments. In the present study, the deficit in skill set for collection of data, inadequate knowledge of research methodology, and Difficulty in performing statistics were the main barriers to research. Personal barriers are more prevalent than regulatory and administrative barriers. More research to include all the staff members in the College and the whole University is needed.