In spite of recent advances in iron overload, elevate iron deposition in pituitary gonadotropic cells keep on one of the main complications in thalassemic patients. Hypogonadism, mainly hypogonadotropic hypogonadism, is usually detected during puberty. Early diagnosis and treatment are crucial for normal pubertal development and to reduce the complications of hypogonadism. The risks and benefits of hormonal replacement therapy, especially regarding the thromboembolic event, remain a challenge for providers caring for thalassemic patients. To assessment correlation between hepcidin and FSH in β-thalassemia major. There were 138 participants in the research study,48 control persons and 90 β thalassemia major patients. Hepcidin and follicular simulating hormone (FSH) values were calculated in order to evaluate correlation between them and calculated levels were estimated using enzyme-linked immunosorbent assay(ELISA) kit. The levels of Hepcidin and FSH in the patients group and in the normal control found to differ significantly (0.039) and (p < 0.001) respectively. Significant moderateNegative correlation was seen between the level of hepcidin and FSH (p < 0.003). Significant moderate negative correlation between hepcidin and FSH in beta thalassemia major make hepcidin good early predictable marker for Hypogonadism. hypogonadism resulting from iron deposi-tion in the pituitary gonadotrope is commonly found in thalassemia major patients. Early diagnosis and treatment are crucial for normal pubertal development and to reduce the complications of hypogonadism.