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Abstract : Neonatal sepsis is the main health problem that is accountable about 30-50% of the overall neonatal mortality in the developing nations. Nurses can provide a straight care and have the ability to impact on neonate's outcomes, so that, teaching nurses about sepsis is measured essential to improve neonate's care safety and outcomes. The present study aims to determine the effectiveness of the educational program in improving nurses' practice and practical procedures that related to neonatal sepsis in neonatal intensive care units. A quasi-experimental design was used. The interventional group included 23 nurses from Heevi pediatric hospital (Neonatal intensive care unit, Semi intensive care unit) and the control group were 25 nurses from maternity hospital, Neonatal intensive care unit. The overall period was 1 year including preparation of the educational program, sampling, analyzing of the results and the discussion. The pre-assessment of the outcomes showed that the control and experimental groups were comparable in practice score, mean and standard deviation 3.17 (0.491), 3.0 (0.5) respectively whereas the post assessment of the outcomes has been showed a highly significant improvement in the experimental practice score in comparison of the control group 3.22 (0.422) 3.96 (0.2). The control and experimental groups were comparable in practice and this comparability is highly significant in interventional group (P-value < 0.000). Maximum number of the participants (52.1%) were in neonatal intensive care unit at maternity and delivery hospital, 26-30 years old was the prevalent age, institutes graduation nurses were the prevalent (54.2%), the majority of nurses (68.8%) their experience was<5 years’ in neonatal intensive care units, (79.2%) of nurses had 1-3 educating and training courses related to neonatal care.

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