Hepatitis B virus infection is the main risk factor for hepatocellular carcinoma worldwide. Hepatitis B virus directly leads to carcinogenicity (cancer) through integration processes in the human genome. This study aimed to accurately characterize the activity of lipoxygenase in hepatitis B patients, ages (20-70) and its relationship to some antioxidants in the body and age. The study showed a significant increase in the activity of LOX enzyme in patients with hepatitis B virus compared to the control group. The concentration of GSH glutathione, as well as an increase in the concentration of Malone Dialdehyde, which is one of the products of oxidation of unsaturated fats, which is the basis for the enzyme LOX lipoxygenase.