The aim of the study was to evaluate the effect of surface treatment on micro-tensile bond strength between natural dentition and ceramic using two different luting agents. The extracted premolars were collected and cleansed of debris by placing them in 1% hydrogen peroxide solution and stored in 0.5% Chloramine T at room temperature and the teeth were embedded in the auto-polymerized acrylic resin. A total of 40 samples were made and divided into Group-I (self-adhesive) and Group-II conventional luting agents. Each group is again subdivided into A-control (no surface treatment), B-acid etching (5%HF), C-sand Blasting (Al2O3 110 μm), D-both acid etching and sandblasting and 5 samples were made in each group. The micro-tensile bond test was performed with a universal testing machine at 1 mm/min cross head speed. The values were analyzed using Mann-Whitney U Test and Kruskal-Wallis Test. Mann-Whitney U Test showed the comparison between Group-I and Group-II with significant value P 0.223 which reveals that there is no significant difference between two Groups and Kruskal-Wallis Test for comparison between the subgroups showed the significant value P <0.001, hence there was significant difference between the subgroups. Hydrofluoric acid etching produces more micro-tensile bond strength between ceramic and teeth than any other surface treatments for both conventional and self- adhesive luting agent.