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Abstract : A four medicinal leaves plants were gainted from local market and the alcohol extraction were doing using 75% methanol. Green silver nanoparticles synthesis from foure medicinal plant extracts. Scanning electron microscopy (SEM), UV visible spectroscopy, Fourier Transform Infrared Spectroscopy(FTIR), and Energy Dispersive Spectroscopy (EDS). The results showed nanoparticles, smooth spherical nanoparticles ranging in size from 29.03 to 88.00 nm. The standard concentration of aflatoxin B1 is (29) ppb, according to the findings of a study on the effectiveness of green nanoparticles against aflatoxin B1 using high-performance liquid chromatography technology to assess its concentration. The extracts were examined by HPLC, and found that the concentration of aflatoxin B1 is The present in the alcoholic extracts. (Thymus vulgaris: 0.065ppb, Rosmarinus officinalis:0.580ppb, Mentha spicata:0.377 ppb, and Eucalyptus camaldulensis:1.067ppb). But Ag nanoparticles alcohol extracts (T. vulgaris:rare, R. officinalis:0.065ppb, M. spicata:1.083 ppb, and E. camaldulensis:0.615ppb). The remove rate in green Ag nanoparticles of alcohol extract showed aflatoxin B1 detoxification ratios by using rosemary: 99.67 % eucalyptus: 96.92 %, mint: 94.58%, and thyme: 92.07%. However, that alcoholic extracts of thyme: 99.67%, mint: 98.15%, rosemary: 98.15% and eucalyptus: 96.49%. From the results appeared the superiority of the thyme plant detoxification over the rest of the plant extracts while rosemary Ag nanoparticles have higher in aflatoxin B1 detoxification activity than other nanoparticles. The mint plant showed less effectiveness against aflatoxin B1.

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