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Abstract : The study aimed to manufacture silver nanoparticles (AgNPs) biological by aqueous extract of broccoli (Brassica oleracea var. italica) and evaluation of its effectiveness against some types of toxin-producing fungi. Identification of silver AgNPs nanoparticles formed by aqueous extract of flowers broccoli via a spectrophotometer UV-vis, Fourier transform infrared FTIR, Field-emission scanning electron microscopy (FESEM), atomic force microscopy (AFM) and X-ray diffraction (XRD(. The result of the UV-Vis spectroscopy showed a peak absorption at the wavelength of 420 nm, while the result of FTIR spectroscopy was the appearance of six stretches different bands that have a role in reducing Ag+ silver ions to Ag0 silver nanoparticles as for FESEM microscopy, it showed that the spherical shape of silver nanoparticles is the dominant and dimensions ranged between 14-44 nm, while the AFM microscope showed two- and three-dimensional, the surface of silver nanoparticles while XRD showed four prominent crystal peaks at 2° theta levels (111, 200, 220 and 311). Silver nanoparticles showed their antifungal activity against two isolates of Aspergillus flavus and Aspergillus niger, where the diameter of the inhibition zone reached (20 ± 0.57, 18 ± 0.57) mm, respectively, by the well diffusion method. The microtiter plate method was the minimum inhibitory concentration of the above two isolates (512 ± 0.00,16 ± 0.00) μg ̸ ml, respectively. The results showed the effectiveness of silver nanoparticles against the mycotoxin aflatoxin B1 secreted mediated by the two isolates of the fungus A. flavus and A. niger as its concentration decreased in the medium of sucrose potatoes liquid PSB to 34.8 ± 0.04 and 0.52 ± 0.01 ppb for both fungi respectively after treatment with these minutes after If its concentration in minutes before treatment was 115.6 ± 0.14 and 12.4 ± 0.11 ppb and the percent of AFB1 reduction 85.5 ± 0.06 % and 8.2 ± 0.11 % also for both fungi, respectively.

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