Abstract : Despite the development in fertility treatments, the prevalence of chromosomal abnormalities remains a crucial indicator of implantation and fertilization rates. Recently, the application of PGD following IVF or ICSI has been widely debated on the incidence of aneuploidy; however, the effect of these methods on chronic abnormalities is yet unknown. Hence, this study aimed to evaluate and identify the effect of insemination methods (ICSI versus IVF) on the incidence of chromosomal abnormalities. In this retrospective cohort study, a total number of 202 women attending Al Manar IVF center and undergoing fertility treatment between 2016 and 2021 were enrolled. The overall number of participants was allocated to 74 women who had performed IVF, and 155 women who had undergone ICSI. Following IVF or ICSI, the preimplantation genetic diagnosis was performed via trophectoderm biopsy. A total of 589 embryos were yielded, out of which n= 165 were obtained from IVF and n= 424 from ICSI. The occurrence of aneuploid embryos was higher in IVF embryos in comparison to ICSI embryos (74% versus 71%). The percentage of different chromosomal abnormalities that involved trisomy 18 was higher in IVF versus ICSI (23.1% vs 18.1%). However, the incidence of trisomy 13 was similar between both groups (28.2% vs 28.3%). Sex chromosome abnormalities were lower in the IVF group as opposed to ICSI (18.7% vs 20.9%). The rate of aneuploid embryos was higher in ICSI compared to conventional IVF. Based on the findings of this study, conventional IVF is the preferred insemination method in PGD cycles.