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Abstract : The study aimed to manufacture silver nanoparticles (AgNPs) biological by aqueous extract of broccoli (Brassica oleracea var. italica) and evaluation of its effectiveness against some types of pathogenic bacteria. Identification of silver AgNPs nanoparticles formed by aqueous extract of flowers broccoli via a spectrophotometer UV-Vis, Fourier transform infrar FTIR, Field-emission scanning electron microscopy (FESEM), atomic force microscopy (AFM) and X-ray diffraction (XRD(. The result of the UV-vis spectroscopy showed a peak absorption at the wavelength of 420 nm, while the result of FTIR spectroscopy was the appearance of six stretches different bands that have a role in reducing Ag+ silver ions to Ag0 silver nanoparticles as for FESEM microscopy, it showed that the spherical shape of silver nanoparticles is the dominant and dimensions ranged between 14-44 nm, while the AFM microscope showed two- and three-dimensional ,the surface of silver nanoparticles while XRD showed four prominent crystal peaks at 2° theta levels (111, 200, 220 and 311). Silver nanoparticles showed their antibacterial activity against Gram-positive bacteria isolates Staphylococcus aureus and Escherichia coli, where the diameter of the inhibition zone reached (22 ± 0.57, 14 ± 0.57) mm, respectively, by the well diffusion method. The microtiter plate method was the minimum inhibitory concentration of the above two isolates (128 ± 0.00, 64 ± 0.00) μg ̸ ml, respectively. Silver nanoparticles have also been shown to have antibiofilm activity for isolate S. aureus where the biofilm formation status changed from (strong) to (moderate) while in the E. coli isolate, it changed from (moderate) to (weak).

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