A total of 170 samples of (chicken poo and sewage) were grown on selective medium for Salmonella isolate identification and bacteriophage isolation (salmonella bacteriophage). Out of 170 samples results gave us the existence of 20 bacteriophage. Salmonella Bacteriophages were found in 11.7% of the samples analyzed and tested against the most commonly isolated of Salmonella isolates. Bacteriophage demonstrated potent lytic activity against Salmonella isolates (enteritidis, typhimurium) by forming a clear and transparent zone in the spot and double layer agar assays, implying that bacterial challenge assays were used to assess the relative ability of Bacteriophage to lyse Salmonella in vitro, producing plaques 3-6 mm in diameter surrounded by halos on a Salmonella lawn. Also using Transmission Electron Microscope to note the shape of the bacteriophage and then diagnosed to which family it belongs, which turned out to be back to the Myoviridae family, revealed the existence of an icosahedral head, 140.20 nm, and a contractile tail, 115.25 nm. Salmonella bacteriophage might encode genes inv-A, sit-C, inv-F from our Salmonella isolates, according to our findings.