Nipple discharge (ND) is a common presentation in breast medicine and comes as the third reason for consultation after breast pain and palpable masses. The aim of the current study is to determine the independent association of prolactin, T3, T4, TSH with female characteristics, clinical prognosis, etc. in a group of affected women with ND syndrome. A retrospective descriptive study on the clinical and laboratory results of prolactin, T3, T4 and TSH hormones data for year of 2020 were obtained from the department of obstetrics and gynecology of Al-Sadr hospital/ Basra/ Iraq. The levels of these hormones were deteremined by ELISA technique. Low level T4, T3 and TSH occurred in 64.7%, 15.7%, and 21.6% respectively. Elevated prolactin occurred in 70.6% of tested females while the rest showed normal level. There was association between low level of TSH and hyperprolactinemia as the associated OR was 5.3 times. T3 and TSH results were significantly high in the all age groups (2.43 mean difference) and a P. value of >0.05 while T4 was significantly high in the (60-69 yrs) subgroup and low in the subgroup <20 yrs. Generally the highest mean difference was seen in the T4 (75.07) while the highest mean difference for the three hormones was seen in the (60-69 years) subgroup. Most of the ND patients had hyperprolactinemia with overt hypothyroidism, as well as in those with subclinical hypothyroidism. ND is partly a hormonal disorder, and there is a close association between the hormones of T3, T4 and TSH and ND. However, there was no independent association between prolactin, T3, T4, and TSH with female characteristics, breast tumors or clinical diagnosis.