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Abstract : Two hundred and fifty specimens (stool) from children under two years for both sexes were collected from some Iraqi hospitals. All isolates were diagnosed according to morphological characteristics, biochemical tests, and results showed, 210 (84%) samples were identified as E. coli, (5 isolates) were identified as Enterohemorrhagic Escherichia coli (EHEC)serotype O157:H7. The identification was confirmed by VITEK 2 system. Susceptibility test was determined for all pathogenic E. coli isolates to 16 different antibiotics, the result showed that 100%resistant to (Ampicillin, Cefazolin, Ceftriaxone, Cefepime, Trimethoprimand Ceftazidime), but Nitrofurantoin, Cefoxitin and Gentamicin were 66%, 40%, 15% respectively, however100% of isolates were sensitive to Piperacillin, Ertapenem, Imipenem, Amikacin, Ciprofloxacin, Levofloxacin and Tigecycline. Monoplex pattern of PCR was used for detection 16SrRNA, eae, stx1,stx2,lifAand Ler genes in E. coli O157:H7, result showed isolates were positive for 16SrRNA,eae,lifA, stx1 and regulatory gene (Ler),while no bands appear ofstx2.

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