Fluid and electrolyte imbalances are the most prevalent dialysis complications. As a result, the completion of this research prepares the method via which dialysis nurses care for dialysis patients in accordance with the most recent advances in nursing interventions and enable them to provide a series of instructions to educate patients and allow them to improve their mental and physical abilities and provide them with knowledge of health procedures followed to reduce fluid and electrolyte imbalances and improve patients' ability to Live a normal life. The study aimed to assess nurses' knowledge of electrolyte imbalances and fluid, and to determine the effectiveness of an educational program on their knowledge of fluid and electrolyte differences. The study design is a quasi-experimental design by applying the two groups in the pretest and posttest periods. The study was conducted in Al-Hussein Teaching Hospital from (December 5 / 2019 to January 5 / 2022). The non-probability sample was selected and included (54) nurses working in the Haemodialysis center. Data were collected using a questionnaire regarding dialysis nurses' knowledge of electrolyte imbalance and fluid. The reliability of the questionnaire was selected using Cronbach's alpha which is (0.972) and Pearson's test (test and retest) which is (0.996). Data analysis was performed by applying descriptive statistics (frequency and percentage) and inferential statistics. The research's findings suggested that now the study group of dialysis nurses benefited from implementing the educational program and adequately improving and developing their knowledge. The level of knowledge of the nurses about electrolyte disturbance and fluid of the dialysis patients of the study group was poor in the pretest before the implementation of the educational program, while it increased to a high level once the instructional program has been implemented. The following suggestions were made by the researcher: Expanding nurses' knowledge and Skills by creating scientific videos on electrolyte and fluid abnormalities in dialysis patients and using these films at various levels of education. Conduct additional studies on the implementation of educational programs on nurses' knowledge and practice about dialysis complications. Skillfully train caregivers working in a specialized dialysis center or develop appropriate health education.