The experiment was conducted for the 2019-2020 growing season. This study aimed to study the effect of spraying of vitamin B3 and the amino acid glycine and their interference on some growth indicators of celery plant; it is included the using three concentrations of the vitamin (0.75,150) mg.L-1 and three concentrations of glycine (0,50,100) mg.L-1. The experiment was designed according to Randomized Complete Blocks Design (R.C.B.D.) with three replicates for each treatment. The averages were compared using the least significant difference (LSD) at a probability level (0.05), and the following results were revealed. 1- Vitamin B3 at different concentrations resulted in a significant increase in all the studied features, as the concentration of 75 mg.L-1 of the vitamin effect on plant height and number of leaves, while the concentration 150 mg.L-1 effect on root length, nitrogen rate, Phosphorous rate, magnesium rate, iron content, leaf content of chlorophyll and protein content. 2- The results indicated that the effect of the amino acid glycine at different concentrations caused a significant increase in all plant growth indicators (plant height, root length, number of leaves, nitrogen rate, phosphorus rate, magnesium rate, iron rate, leaf content of chlorophyll and protein rate. 3- The effect of the overlap between the two study factors was significant in all the studied traits, as the combination between 75 mg.L-1 of vitamin and 50 mg.L-1 of glycine has greater effect on plant height and number of leaves, while the combination between 150 mg.L-1 of vitamin and 50 mg.L-1 of glycine in was with greater effect on root length, nitrogen rate, phosphorous rate, magnesium rate, iron rate, leaf content of chlorophyll, protein rate.