Antibiotics have great benefits, however, not free from side effects. Its side effects, especially those related to liver damage and increased levels of liver enzymes, have prompted the world to search for a traditional medicinal plant such as Rutin to find preventive and curative agents against multi-organ dysfunction (unwanted effects) of Ciprofloxacin. The aim of this study is to evaluate the preventive and therapeutic effect of Rutin in inducing liver injury in animals by Ciprofloxacin. The study included Six groups, each group contains 5 male albino rats and the dose was taken orally daily, The control group were given normal drinking water for 14 days, While The first treatment group (T1) were given Rutin concentration of 50mg/kg of body weight for 14 days, and The second treatment group (T2) were given Ciprofloxacin antibiotic, and it’s concentration equal 14mg/kg of body weight for 14 days, The third treatment group (T3) were given Rutin concentration of 50mg/kg of body weight for 14days and then Ciprofloxacin oral given with a dosage of 14mg/kg of body weight for 14 days, The fourth treatment group (T4) were given Ciprofloxacin antibiotic concentration of 14mg/kg of body weight and then Rutin oral given with a dosage of 50mg/kg of body weight in combination for 14 days and The fifth treatment group (T5) was given Ciprofloxacin antibiotic concentration of 14mg/kg of body weight for 14 days and then Rutin with concentration equal 50mg/kg of body weight for 14 days. histopathological changes occurred in the liver of the animals of the group (T2) represented with Loss of the normal hexagonal arrangement of hepatocytes, cellular dissociation, congestion in a central vein, fatty necrosis, and lymphoid infiltration compared with other groups. Rutin treatment at various doses has hepatoprotective effect by maintaining the functional integrity by improving the cellular antioxidant status, reducing oxidative stress, and preserving the integrity of the histomorphological structure of the liver. It was concluded that Rutin at 50mg/kg has an important hepatoprotective effect against injury induced by Ciprofloxacin in the liver.