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Abstract : Klebsiella pneumoniae (100 isolates) were isolated from different clinical sources and showed resistance towards eight antibiotics and exhibited ability to form biofilm. Binding of ZnO-NPs with K. pneumoniae Lipo-Polysaccharide determined by UV-VIS spectrum and FTIR analysis. Biological activities were determined to LPS- ZnO NPs and showed Antibacterial activity with high inhibition zone (31.8mm) against K. pneumoniae, while antibiofilm activity showed a higher inhibition percentage (95%) for Pseudomonas aeruginosa. The antitumor activity showed that LPS- ZnO NPs devement the cell viability of breast cancer cell line (MCF-7), and this decrease related strongly to the concentrations significantly (P < 0.05). The percentage of decrease viability was 45±4.3, 50±2.5, 62±3.0, 71±1.1, and 83±1.4 in concentration 400, 200, 100, 50, and 25 µg ̸ mL respectively. Also, LPS and ZnO-NPs showed a significant effects of viability rate to the MCF7 cell line ranged as 68±6.0,85±3.0,95±1.0,96±1.1and 94±1.5 and 52±4.1, 63±2.1, 77±2.4, 90±1.8, and 95±0.5 respectively. In comparison to results of cytotoxicity of each ZnO NPs, LPS and LPS- ZnO NPs did not showon significant differences (P < 0.05) in the inhibition of viability rate of the normal cell line (WRL 68).

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