About the Journal

Download [This article belongs to Volume - 140, Issue - 01]

Abstract : L-lysine is one of nine necessary amino acids for human and animal nutrition. In animals, Llysine is considered necessary and must be supplied from dietary sources. Two hundred and fifty clinical specimens were collected from different clinical sources. Twenty-two isolates (8.8%) were identified as Staphylococcus aureus. The ability of S. aureus isolates to produce lysine was examined qualitatively and quantitatively. The results showed that all isolates under study had the ability to produce lysine at different concentrations. The optimal conditions for the production of lysine were tested, in which 37 °C was the best temperature. The optimum pH was 7.4. The optimum incubation period for lysine production was 72 hours. The purification was done using affinity ion exchange chromatography on amberlite IR-120. The total amount of purified lysine was 473 mg/L. The effect of lysine on human blood clotting (13 coagulation factors) may be on coagulation factors (XII, XI, IX, VIII, and X) of the intrinsic coagulation pathway. Also, lysine may be on extrinsic coagulation factors (VII) and common coagulation factors (X, V, 1, and II), inferred by PT, INR, PTT, and fibrinogen tests.

Copyright © 2020 All rights reserved | Bulletin of National Institute of Health Sciences