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Abstract : Urinary tract infections (UTIs) are common in bothdiabetes &non-diabetic people alike. Because the germ that cause illness differ in their susceptibility to antibiotic from region to region and, it is necessary to monitor trends and susceptibility patterns of the most common organisms to antimicrobials on a regular basis. The goal of the study is to establish the uropathogen spectrum and antibiotic sensitivity pattern in individuals with clinically diagnosed UTI, both diabetic and non-diabetic. This study began from the january, 2020 till the mid of january, 2022. A total of five hundred (500) Diabetic &non-diabetic patients' urine samples were obtained. These were collected from patients of both sexes and different ages hospitalized in salah-aldin Hospital. our study show that increase frequency of urinary tract infection in diabetic subject 148/250(59.2%) in comparision to control 117/250(46.8%). The isolates were identified according to morphological, cultural and biochemical characteristics and confirm by Vitek-2 compact. There is no asignificant difference in the causative agent between diabetic &non-diabetic patients, Most prevalence symptoms among diabetic and non-diabetic was fever, dysuria, abdominal pain, vomiting, pyuria and hematuria, most causative agent of UTI was E.coli (40.5%) diabetic (47%) nondiabetic followed by K.pneumonia (23.6%) diabetic (22.2%) nondiabetic, S.aureus (12%) diabetic (17.9%) nondiaetic, P.aeruginosa (15.5%) diabetic (7.6%) nondiabetic, P.mirabilis (4.7%) diabetic (5.1%) nondiabetic and E.faecalis (3.3%) only within diabetic patients.

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