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Abstract : Invasive aspergillosis remains a severe fungal disease with high mortality. In this study, we isolated variant strains of Aspergillus fumigates from different sources, then those strains were stressed through exposing to ultraviolet radiation(UV). Moreover, these wild strains allowed to grow on nutrient deficient media and also the variants incubated at different temperatures (15°, 25°,45°C) .After that we developed intranasal mice model of invasive pulmonary aspergillosis (IPA), in which groups of mice immunosuppressed by cortisone acetate then mice divided into groups in which some groups for infection by the wild isolated strains ,other for infection by the stressed strains .Depending on mean cut off value for serum galactomannan test ≥.5 ODI, the GM antigenemia of serum from mice group infected with wild variant strains was positive in 16/20 tested samples (78%) with considerable variation of GM- index (ODI. 61: 2.39), also lung tissue from these mice showed positive growth culture with fungal invasion and obvious lesions using PAS staining .On the other hand , the GM index of serum from mice group infected with UV exposed variant strains and strains grown on nitrogen & carbon deficient media tested negative GM test with ODI < .5, also fungus not recovered from their lung tissues during culture .Serum samples of the mice group infected with variant strains incubated at 25°C and 45°C tested positive with varied ODI, and lung tissue from these mice showed positive growth culture with lesions using PAS staining .Our conclusion from that results was that the stressed strains showed difference on virulence in comparison to the wild isolated strains.

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