Archive of
Bulletin of National Institute of Health Sciences
Title : Role of H.Pylori and TNF-alpha in some patients with Type-1 Diabetes Mellitus.
Abstract :This Study was conducted in Diyala Governorate/ Iraq. For a patient with type-1 Diabetes, this Study aimed to evaluate some of the cellular kinetics that causes inflammation in the pathogenesis of type1 Diabetes by measuring the level of TNF-a, as they were determined using ELISA technology (sandwich Enzyme _Linked Immunosorbent Assay). After that, H.pylori bacteria were added to this research. The aim was to determine the relationship between Helicobacter pylori and its association with type1 Diabetes by measuring this bacteria's level of globulin (IgG, IgM) and knowing the immunopathology. Sixty samples were collected from type 1 diabetes patients after diagnosis. According to the criteria approved by the world Health Organization for diagnosis of type 1 diabetes, the number of males was (35), and the number of females was (25). Forty blood samples were taken from apparently healthy people, which were adopted as a control group if the number of males reached (20), and the number of females was (20). The level of cellular kinetic that was measured in this Study was determined; the results showed an increase in the level of Tumor necrosis factor-alpha (TNF-a) among the group of patients (234.9406±121.753361) pg\ml compared to the healthy group (62.4782±18.21447) pg\ml.
Title : ROS and cardiac function test in type 2 diabetes mellitus after Pfizer vaccination among Iraq patients
Abstract :Vaccination in diabetics is important to prevent cardiac complications that happen in type -2 diabetes mellitus after COVID-19 infections. Measurement of Reactive Oxygen Species (ROS) as indicators of oxidative stress in T2DM patients. and measuring variable of cardiac function test (Troponin-I, creatine kinase CK-MB) after the second dose of Pfizer vaccination. ROS (Reactive oxygen species) and cardiac function tests (Troponin-I, CK-MB) were determined by using an ELISA kit. The results revealed a higher level of ROS in both group of diabetic in comparison to controls elevated in diabetic (slightly higher in diabetics non vaccinated than vaccinated) I compare with healthy controls lower, while cardiac function tests including Troponin-I higher in diabetics (vaccinated higher than non-vaccinated) in compare with healthy control.CK-MB higher in diabetics and healthy vaccinated in compare with diabetics non vaccinated. ROS not changed after Pfizer vaccination. Vaccinated type 2 diabetes show marginal elevated in Cardiac function test and ALL Cardiac function test mean with normal range (diabetics show higher level of Troponin-I in compare with healthy control) while CK-MB higher in diabetics vaccinated and healthy control in compare with diabetics non vaccinated.
Copyright © 2020 All rights reserved | Bulletin of National Institute of Health Sciences