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Bulletin of National Institute of Health Sciences
Title : The Effect of Follicular Fluid Zinc Concentration On Oocytes Maturity and Embryo Quality in Subfebrile Women with Polycystic Ovary Syndrome
Abstract :PCOS is one of the most common metabolic endocrine disorders affecting women of reproductive age. Zinc, is important trace element in the body. It is required for "DNA, RNA, and protein synthesis, cell-mediated immunity& reproduction". "Lack of antioxidants and oxidative stress" may negatively influence oocyte quality and conception rate. Is to measure the zinc contents in the follicular fluid of infertile PCOS women having ICSI in order to assess oocyte number and quality. A cross-sectional study was conducted on 80 subfertile polycystic ovarian women between the ages of 20 and 40 who were referred to the reproductive clinic at ALSadder teaching hospital and had Intra Cytoplasmic Sperm Injection (ICSI). Determine the zinc concentration in follicular fluid. In addition, oocyte maturation and embryo quality were evaluated using inverted optical microscopy. Follicular fluid zinc does not have a significant correlation with metaphase I (MI)" and "germinal vesicle", but it has a substantial positive correlation with the number of total oocytes, MII oocytes, total embryos, and Grade I embryos. GI embryo numbers and the amount of FF zinc in women's ovaries who were candidates for ICSI treatment and who had a history of PCOS were shown to be highly correlated. This was also true for the number of oocytes and the quality of those oocytes.
Title : Individualized Luteal Phase Support Based on Serum Progesterone level P4 prior to Frozen Embryo Transfer Cycles FET and Pregnancy Outcome
Abstract :The problem of an excessive number of embryos being produced now has only one remedy, and that is the cryopreservation of embryos. Even though fresh endometrial tissue is typically used in assisted reproductive technologies (ART). To evaluate if progesterone P4 monitoring prior to and on the day of frozen embryo transfer can accurately predict pregnancy outcome. This is a cross-sectional observational study of 50 infertile women. Follow-up was done until the foetal heart was positive. Blood samples were obtained the day before and the day of embryo transfer in frozen cycles to assess serum progesterone p4. One day before embryo transfer, samples were obtained. Endometrial preparation uses standardised HRT. The pregnancy rate among all infertile women who participated in the trial was 35.7%. Age, BMI, infertility etiology, and embryo grade were not associated with hormonal monitoring. Individuals with low blood P4 levels the day before FET may benefit from daily intramuscular P4 injections if they are started the day before FET, and their reproductive success is comparable to that of patients with appropriate P4 levels to begin with. Prior to embryo transfer, monitoring progesterone levels revealed a substantial difference, with a P4the threshold level of 11.6ng/ml. Monitoring blood progesterone levels the day before and the day of embryo transfer improves pregnancy chances. Individualized luteal phase support (Ilps) provided the day before embryo transfer may increase the chance of a successful pregnancy.
Title : The Impact of Covid 19 Infection and Vaccine on Ovarian Reserve in Reproductive age Women in Iraq
Abstract :Coronavirus illness (COVID 19) developed in China and spread over the globe. The reproductive system is a high-risk target for SARS-COV 2 infection and countrywide immunisation with Pfizer-BioN tech and other vaccines. No proven proof of illness and vaccine's influence on future fertility. To assess the possible effect of COVID 19 infection and vaccine on ovarian reserve in reproductive age women in Iraq. A case-control research conducted at Kamal Al-Sameraie hospital for infertility and IVF, Al- Nada specialised private centre for IVF, Baghdad Iraq, and Al Najaf infertility and IVF facility, Al Najaf Iraq, from June 2021 to June 2022. 150 infertile women of varying ages are randomly separated into infected, vaccinated, and non-infected non-vaccinated groups. More over two-thirds of infected individuals had mild COVID-19 infections, but only 36% of vaccinated patients had mild reactions. AFC, serum FSH, and AMH levels in the vaccinated group were (10.664.46, 6.963.23, 1.821.03) whereas in the control group they were (126.64, 6.262.23, 2.512.44) with no significant difference (P>0.05). There was also no significant change in ORT (AFC, FSH, AMH) between infected and control groups. Serum AMH levels in the infected group were (2.051.46) before infection and (1.871.42) after infection (P=0.5). There was no significant change in the vaccinated group before and after immunisation (P=0.4). No significant changes were identified in COVID-19 infected group ovarian reserve markers (P>0.05). Covid 19 infection and vaccine did not appear to affect ovarian reserve, function and future fertility in reproductive age women.
Title : Effect of embryo transfer depth on IVF/ICSI outcomes
Abstract :In spite of the notable advancement in the assisted reproductive technology, implantation failure still a considerable issue in the majority of infertile couples getting these treatments. Embryo transfer is important in assisted reproductive technology and has agreat effect on the implantation rates and pregnancy outcomes. To assess the effect of two different distances of embryo transfer site from fundal endometrial surface on the outcomes of intracytoplasmic sperm injection (ICSI) cycles. A total of 120 women who were candidate for ICSI/ embryo transfer in High Institute for Infertility Diagnosis and Assisted Reproductive Technologies / Al-Nahrain University and and infertility center at AKamal Al Samaraay hospital in Baghdad during the period from December 2021 to August 2022were equally assigned to two groups based on the distance between the fundal endometrial surface and catheter tip to investigate implantation, chemical and clinical pregnancy (group A:(10 -15) mm and group B: (16 – 20) mm, respectively). The patients in the group B had significantly higher implantation rate, chemical and clinical pregnancy rate as compared to the group A (p = 0.005, 0.03, 0.02, respectively). The rate of miscarriage had no significant differences between groups (p=0.3). In conclusion, the present study demonstrated that the depth of embryo deposition inside the uterine cavity at a distance of (16-20) mm below fundal endometrial surface have better effects on the pregnancy outcomes of ICSI cycles and can be considered as an important factor to improve the success of IVF cycles.
Title : Toll-like receptors 4 antagonist, Ibudilast, ameliorates acute renal impairment induced by sepsis in an experimental model.
Abstract :In critically ill patients, acute kidney damage (AKI) is a frequent consequence that is linked to higher morbidity and mortality. The most typical cause of AKI is sepsis. Ibudilast is a strong inhibitor of phosphodiesterase (PDE)-4 and other PDE subtypes as well as a TLR-4 antagonist. To examine the nephroprotective effect of Ibudilast by their anti-inflammatory, antioxidant effect and modulation of apoptosis through their effect on TLR- 4/NF- kB signaling pathway in experimental model of sepsis. 24 adult male mice divided randomly in to 4 groups (Sham group, cecal ligation and puncture (CLP) group, Vehicle group, Ibudilast (10 mg/kg) treatment group). We sacrificed the animals after 24 hours, and each mouse's blood sample was drawn. To identify markers (tumor necrosis factor alpha (TNF-α), interleukin- 6 (IL-6), macrophage migration inhibitory factor (MIF), interleukin- 10 (IL-10), F2-isoprostane, caspae-3, B-cell lymphoma-2 (Bcl-2), toll like receptor- 4 (TLR-4) in kidney homogenate. Part of the kidney was studied for histopathology. Ibudilast significantly reduced TNF-α, IL-6, MIF, induced IL-10, lowered F2-isoprostane, dropped caspae-3, rose Bcl-2, lowered toll like receptor- 4 protein expression, reduced blood urea and reduced serum creatinine. Ibudilast lessens kidney damage induced by sepsis through blocking TLR-4/NF-kB downstream signal transduction pathways, oxidative stress and modulation of apoptosis.
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