Because the seeds of most plants, including the seeds of cress, contain a high level of proteases, therefore Purify the enzyme from the seeds of garden cress in several steps, including concentration with ammonium sulfate at a saturation rate of 50-60%. The specific activity of the enzyme reached 61.513 U / mg protein. Then the enzyme was passed through an ion exchange DEAE-cellulose column. The enzyme recorded a specific activity of 132,080 units/mg protein, with a purification fold 2.147, and yield 30.49%. Then gel filtration on a Sephacyl S-200 column. The enzyme recorded specific activity, purification fold and its yield, 660 U/mg protein, 4.1641, 27.72% respectively. The enzyme was characterized by setting the optimum pH and optimum temperature. The enzyme recorded the highest activity at pH 7 and pH stability from 7-8. The enzyme also showed the highest activity at a temperature of 35°C for a period of 15 minutes. The enzyme was also showed stable at a temperature 20-40°C for 15 minutes. The result of the effect of chemical compound on the activity of the enzyme showed that it was not affected by the present of sodium and potassium chloride except in a small percentage at concentration 5,10 mM, but it increase slightly when the enzyme was incubated with calcium chloride, it reached 104,110 and magnesium chloride 110,115 at concentration 5,10 mM, respectively. No effect was shown for thiol compounds such as cysteine at concentration 1,10 mM while it was affected by the presence of chelating agents EDTA. Also studied the effect of the activity of the enzyme purified from cress seeds against the types of positive and negative gram stain, as well as fungi and yeast, the result showed the activity of the enzyme at concentration 10,15 and 20 and that the highest response of the bacteria was at 20 concentration, while, no effect of fungi was shown except at concentration 20.
Recently, p-Coumaric acid was investigated in diabetic rats. Therefore, the purpose of this study was to determine whether p-Coumaric acid could reduce oxidative stress in diabetic rats. As a result, STZ and p-Coumaric acid were obtained from an American academic research institute in Iraq for this investigation (TAARII). The researchers induced diabetes in rats, giving STZ dissolved in buffer solution. Streptozotocin was given to the treatment group (T1) at a 25 mg/kg body weight dose for 30 days. After 30 days of treatment with p-Coumaric acid at a dose of 100 mg/kg body weight, rounded and broad seminiferous tubules with spermatozoa in their lumen were detected in male rat testes (T2). Streptozotocin and p-Coumaric acid (T3) treated animals with abnormal histopathology. Diabetic animals weighed less than control rats in T1 but more in T2 and T3. The diabetic rats gained a significant weight following a 28-day treatment with 100 mg/kg p-coumaric acid (p0.05). There were significantly more diabetic rats than non-diabetic rats. The effects of p-Coumaric acid on oxidative stress were investigated in diabetic rats using STZ and p-Coumaric acid. According to the study, p-Coumaric acid prevents blood sugar decreases. As a result, in diabetic rats, oxidative stress was decreased; therefore, p-Coumaric acid is anti-diabetic and insulin-resistance. Our research discovered that p-Coumaric acid might aid in the prevention of diabetes.
Late microvascular problems such retinopathy, neuropathy, and diabetic nephropathy are frequently brought on by uncompensated chronic hyperglycemia. In the pathophysiology of diabetes mellitus, the vascular endothelial growth factors (VEGFs) and their receptors (VEGFRs) are of critical importance. The aim of this study to assessment level of VEGFA and VEGFR-2 in a group of patients with un-controlled type 2 diabetes mellitus (T2DM) and compare it with apparently healthy non diabetic subjects and study the correlation with other factors. There were 180 participants in the research study,70 control persons and 110 T2DM patients. Glycated hemoglobin (HbA1c) and fasting plasma glucose (FPG) values were calculated in order to evaluate glycemic management. In addition, serum cholesterol, triglyceride, HDL and LDL were calculated by COBAS INTEGRA 400 plus. VEGF-A, VEGFR-2 levels were estimated using enzyme-linked immunosorbent assay(ELISA) kit. The levels of VEGF-A and VEGFR2 in the patients group and in the normal control found to differ significantly (p < 0.001). Significant positive correlation was seen between the level of VEGF-A and glucose, duration of disease. Weak non-significant positive correlation was also found between VEGFR2 and serum glucose (p=0.09). Patients with uncontrolled diabetes produce VEGF-A and its receptor 2 concentrations that are comparable to those of apparently healthy people, which may be a sign that insufficient glucose management accelerates the development of vascular problems. The absence of correlation between VEGF-A, VEGFR2 and lipid abnormalities demonstrate that lipid problems related to diabetes could not affect how angiogenesis is regulated.
This study irradiated the tin oxide membrane deposited on glass floors at a temperature of 450 ° C. The tin was deposited in a vacuum thermal fumigation method. This makes the membrane more homogeneous than the rest of the methods. The results of X-ray analysis showed that the membrane is multi-crystallized and has access to a crystalline system by Miller coefficients. The pure unirradiated sample was found to be the predominant direction. (111) for silicon and higher intensity in addition to Miller's transactions (-120), (102) and (150) and strictly (60 a.u), (12 a.u), (490 a.u) respectively and note that there is a new angle which is 2Ɵ=25.8786 directionally (-120) After irradiation for three days there was an increase in the height of intensity at the 2Ɵ=25.8786 angles 2Ɵ=44.2498 a decrease in the intensity value at the 2Ɵ=59.1840 angle to (340 a.u). The results of the study of the AFM atomic force microscope showed that the surface before irradiation was poles and at irradiation for a full day its shape shifted from peaks to curves subject to caucasian distribution and after the radiation time increased to three days the surface was rearranged into peaks and the rate of nanoscale dimension (17 nm) was 1day irradiation time.
Favism, which is a severe hemolytic anemia that occurs after ingesting fava bean (FB), has been known since ancient times. In the mid-20th century, a link between this disease and glucose-6- phosphate dehydrogenase deficiency was discovered This study was conducted to Comparative between two method gel filtration chromatography using sephadex G100 and SDS gel electrophoresis for G6PD enzyme purification and the result for this study was shown molecular weight of the G6PD equal 63k.Da for the enzyme extraction using gel filtration technique and 56k.Da by SDS-gel electrophoresis were approximately the same for this method.
Copyright © 2020 All rights reserved | Bulletin of National Institute of Health Sciences