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Bulletin of National Institute of Health Sciences
Title : Association study between acetyl choline esterase and some antioxidative markers in patients with β-thalassemia
Abstract :This study evaluates the association between acetyl choline esterase and some antioxidant markers in the plasma of β-thalassemia patients divided to (85 with β-thalassemia major and 15 with β-thalassemia intermediate) and 50 healthy subjects as control. Antioxidant markers including: total protein, albumin, globulin, albumin/globulin ratio, free amino, free amino / total protein, thiol, thiol / total protein, carbonyl and carbonyl / total protein. The results showed that there was a significant increase in the activity and specific activity of acetyl cholinesterase, total protein, albumin, globulin, thiol, free amino and carbonyl in β-thalassemia patients compared with control group. The results above indicated that these parameters may be good biochemical markers for the evaluation of disease.
Title : Removal of PPD dye from its aqueous solution by Adsorption using the Cadmium oxide nanoparticles
Abstract :The manufacture of Cadmium oxide nanoparticles utilizing a green method is the subject of this research (environmentally friendly). Using the tomato leaf plant's aqueous extract. Using an adsorption batch technique, this oxide was employed to remove tincturepaea-Phenylenediamine from its aqueous solutions. To describe and study the surface, several techniques such as FT-IR, XRD, SEM, TEM, and AFM were applied. All of the reflection peaks with relative intensities of different planes, as determined by XRD analysis, indicate the existence of CdO, and the spectrum revealed that the particle size obtained was around (48.28 nm), which corresponded to those estimated by SEM and TEM. The sizes of the CdO particles were measured using SEM, TEM, and AFM, and they were found to be in the nanoscale range. The influence of contact time, adsorbent, and other variables the thermodynamic parameters for the influence of temperature were calculated after studying the dosage and beginning concentration. And the effect of the change in the acidity function, as well as the effect of increasing the temperature. Through the results, it was found that the best adsorption occurred at a time of 90 minutes and at a quantity of (0.2 g) of the adsorbent substance, as well as at the (PH = 5) the adsorption rate was at its best and at a temperature of (338 K). Recorded the best adsorption rate Among the results obtained when applying the Langmuir and Freundlich isotherms, the adsorption processes were of a physical nature.
Title : Synergic Effect of ZnO- nanoparticles combined with lipopolysaccharide from Klebsiella pneumoniae on cancer cell line
Abstract :Klebsiella pneumoniae (100 isolates) were isolated from different clinical sources and showed resistance towards eight antibiotics and exhibited ability to form biofilm. Binding of ZnO-NPs with K. pneumoniae Lipo-Polysaccharide determined by UV-VIS spectrum and FTIR analysis. Biological activities were determined to LPS- ZnO NPs and showed Antibacterial activity with high inhibition zone (31.8mm) against K. pneumoniae, while antibiofilm activity showed a higher inhibition percentage (95%) for Pseudomonas aeruginosa. The antitumor activity showed that LPS- ZnO NPs devement the cell viability of breast cancer cell line (MCF-7), and this decrease related strongly to the concentrations significantly (P < 0.05). The percentage of decrease viability was 45±4.3, 50±2.5, 62±3.0, 71±1.1, and 83±1.4 in concentration 400, 200, 100, 50, and 25 µg ̸ mL respectively. Also, LPS and ZnO-NPs showed a significant effects of viability rate to the MCF7 cell line ranged as 68±6.0,85±3.0,95±1.0,96±1.1and 94±1.5 and 52±4.1, 63±2.1, 77±2.4, 90±1.8, and 95±0.5 respectively. In comparison to results of cytotoxicity of each ZnO NPs, LPS and LPS- ZnO NPs did not showon significant differences (P < 0.05) in the inhibition of viability rate of the normal cell line (WRL 68).
Title : Identification of Aspergillus Fungi and Detection of Their ability to produce aflatoxin
Abstract :Aspergillus flavus, Aspergillus parasiticus, and Aspergillus nomius are an aflatoxin-producing fungus which is poisonous to humans and animals when consumed. Any step in the manufacture, collection, assembly, transportation, or storage of convenience foods might be contaminated by an aflatoxigenic fungus. Researcher aimed to find out how often aflatoxin and its many forms are in food identified by researchers throughout the globe from 1983 to 2020 using a review and meta-analysis. To find all primary studies on the occurrence of aflatoxins in raw oilseed, a search approach was used. The probe will the last 37 years, from January 1, 1983, to December 31, 2020. The research relied on online resources including Scopus, Web of Science, PubMed, Agris, and Agricola to make sure participants had an appropriate and acceptable level of exposure. Google Scholar and the citations of the research mentioned yielded relevant scholarly literature. The original data was used to infer the existence of aflatoxins if they were not discovered throughout the inquiry. The Metaprop module in STATA was used to calculate the pooling frequency of mycotoxins because to the high number of positive aflatoxins found in raw soybean sample and the large number of cases. There must have been some dispute or conflict in the outcomes of previous studies and official documents, highlighting the need for systematic review and subgroups analyses to reach definite conclusions. Risk evaluations of mycotoxins or aflatoxins in raw oilseed, as well as identifying the maximum output mycotoxins levels in it, are also recommended, especially for nations with a high reliance on oil.
Title : Inhibitory effect of silver nanoparticles synthesized by aqueous extract of broccoli flowers against some species of pathogenic bacteria
Abstract :The study aimed to manufacture silver nanoparticles (AgNPs) biological by aqueous extract of broccoli (Brassica oleracea var. italica) and evaluation of its effectiveness against some types of pathogenic bacteria. Identification of silver AgNPs nanoparticles formed by aqueous extract of flowers broccoli via a spectrophotometer UV-Vis, Fourier transform infrar FTIR, Field-emission scanning electron microscopy (FESEM), atomic force microscopy (AFM) and X-ray diffraction (XRD(. The result of the UV-vis spectroscopy showed a peak absorption at the wavelength of 420 nm, while the result of FTIR spectroscopy was the appearance of six stretches different bands that have a role in reducing Ag+ silver ions to Ag0 silver nanoparticles as for FESEM microscopy, it showed that the spherical shape of silver nanoparticles is the dominant and dimensions ranged between 14-44 nm, while the AFM microscope showed two- and three-dimensional ,the surface of silver nanoparticles while XRD showed four prominent crystal peaks at 2° theta levels (111, 200, 220 and 311). Silver nanoparticles showed their antibacterial activity against Gram-positive bacteria isolates Staphylococcus aureus and Escherichia coli, where the diameter of the inhibition zone reached (22 ± 0.57, 14 ± 0.57) mm, respectively, by the well diffusion method. The microtiter plate method was the minimum inhibitory concentration of the above two isolates (128 ± 0.00, 64 ± 0.00) μg ̸ ml, respectively. Silver nanoparticles have also been shown to have antibiofilm activity for isolate S. aureus where the biofilm formation status changed from (strong) to (moderate) while in the E. coli isolate, it changed from (moderate) to (weak).
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