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Bulletin of National Institute of Health Sciences
Title : Granular cell tumor of the Breast - Case Report and Review of Literature
Abstract :Granular cell tumour (GCT) is a rare tumour that can occur anywhere in the body. The tongue is the most frequent anatomical site of origin, preceded by soft tissues. It is most frequent in 30 to 50 years old females, particularly of African-American ethnicity. This tumour often seems to occur as a firm to hard, non-tender lump that is well confined and unfixed to the surrounding tissues. The tumour almost always occur as a single lesion, but in 5.4 to 17.6 % of patients, presented as more than one lesions. Breast GCTs are formed in intra-lobular mammary connective tissue. Its frequent occurence in upper inner quadrant of the breast reflects the course of the supra-clavicular nerve and the cell of origin of this tumor is considered to be the schwann cells. S-100 protein is highly positive in tumour cells while negative expression is noticed for epithelial membrane antigen, mucin and cytokeratin staining. While the most of GCTs are benign, there have been a few reports of malignant instances (less than 1% of all GCTs, including breast lesions, are cancerous). GCTs can resemble breast cancer on mammography and on clinical examination. Hence, it is important to consider GCT in the differential diagnosis of both malignant and benign breast lesions. We present here a rare case of GCT of the breast in a 62 year old patient alogn with a breif review of literature with an emphasis on the diagnostic characteristics.
Title : Determination of Amlodipine Besylate in its pharmaceutical preparations using spectrophotometric methods
Abstract :Two simple study in this research sensitive, rapid and unexpansive are schiff’s base and oxidative coupling reaction for determination of Amlodipine Besylate (AML) ether in pure form or in it’s tablets. The methods were: (I) Synthesis of a Schiff's base (in basic medium) between (AML) and Benzaldehyde, this form a yellow product which has the highest absorption at λmax (383 nm). (II) Oxidative coupling reaction between (AML) and Phenylhydrazine in basic medium to form yellow product of absorbace at λmax (364 nm). Beer's law is obeyed from (10-165) µg/ml with a molar absorptivity (1.91×105) L/mole.cm. and the limit of detection is (0.02) µg/ml for the first method and (5-150) µg/ml with a molar absorptivity (1.59×105) L/mole.cm. and the limit of detection is (0.02) µg/ml for the second method. In this proposed methods were successfully applied to the determination of (AML) in pure form and in pharmaceutical preprations.
Title : Study Genotyping of cnf ,hly ,aer ,pap genes in Uropathogenic Escherichia coli from Chronic kidney patients
Abstract :In humans, urinary tract infections (UTIs) are still the second most prevalent kind of bacterial infection. They afflict persons of all ages and usually necessitate immediate medical attention. Because of the impaired defense mechanism, Asymptomatic urinary tract infections are more prevalent in hemodialysis (HD) patients with chronic renal failure. The study's goal was to isolate and identify Escherichia coli from chronic kidney patients, as well as to establish the existence and genotype of virulence genes (cnf, hly, aer, pap) in Escherichia coli uropathogens. In this study, 150 clinical samples were collected in total chronic kidney patients. UPEC was isolated on MacConkey and EMB agar, then further identified by a microscopic examination and the VITEK-2 System. Escherichia coli uropathogens were identified using specific primers. The study found 27 (12.0%) CKD patients infected with bacteria and 123 (54.7%) CKD patients not infected with bacteria. Amplification of aer gene found 8 (29.62%) in 27 isolated and hly gene was approximated at 2 (7.40%). CNF gene in E. coli did not found in all isolates. The prevalence of pap virulence genes was found in 6 (22.22%) of the 27 isolates. The registered sequencing of these genes in NCBI under ID were hly gene Lc699243.1, pap gene Lc699244.1, aer gene Lc699245.1.
Title : Powerful antibacterial and anti-biofilm effect of Eco-friendly green synthesis copper oxide nanoparticles on biofilm MDR pathogenic E.coliisolated from clinical cases of animal and human
Abstract :Development of green technology is generating interest of researchers towards ecofriendly and low-cost methods for biosynthesisof nanoparticles (NPs). In this study, copper oxide (CuO) NPs were synthesized using a copper nitrate trihydrate precursorand Ficuscaricaleaves extract as a reducing and capping agent biosynthesized CuO NPs were characterizedusing an X-ray diffractometer (XRD), scanning electron microscopy energy dispersive X-ray spectroscopy (SEM-EDS),transmission electron microscope (TEM), Ultraviolet visible spectroscopy (UV-Vis), and Fourier transform infrared (FTIR)spectroscopy.antibacterial and antibiofilm activity of Cuo NPs were evaluated by agar well diffusion and micro titer plate method respectively Moreover, the antimicrobial activities of different concentrations of CuO NPs synthesized using Ficuscaricaextract were also tested. Accordingly, the outstanding of antibacterial activity of cuo np was the powerful effect with greater inhibitor zone reach to 35 mm where consider a largest one than other green metal oxide nanoparticles. The result revealed a significant difference between the antibacterial effect of green CuO NPs on human and animal E.coli p value=??. CuONPs demonstrated significant antibacterial activity. Hence, they may be used as an antibacterial agent and could be used as an effective alternative to traditional antibiotics.
Title : Estimation the role of inflammation and omentin levels in women with PCOS
Abstract :The study's goal was to investigate the relationship among inflammation parameters and Omentin levels in PCOS patients. The study involved 50 women have PCOS (22 single and 28 married) and 15 women as controls from Salah Aden General Hospital in Salahdin, Iraq, from February 2021 to May 2021 with aged from 20-45 year, levels of omentin, TNFα and IL18 was measured by using ELISA technique. The results showed significant increase in the omentin and TNF- α at (p <0.05) and decreased but not significant in serum IL18.
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