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Bulletin of National Institute of Health Sciences

Journal ID : BNIHS-19-03-2022-10208
Title : Effect of Natural Products of Some Herbs on Biochemical parameters in Rats
Abstract :

Pistacia khinjuk is a plant that has long been utilized in common medicine to cure a variety of conditions. The aim of this study was to evaluate that Pistacia khinjuk fruit extract affected several biochemical markers in a rat model to examine whether it might be used in traditional medicine. The hydroalcoholic extract was prepared by air-drying the plant's fruits. A total of forty male rats were separated into eight groups, with one serving as a control group and the others as test groups. The test animals were given orally for twenty-eight days a 200 mg/kg dose of Pistacia khinjuk for group2, Sorafenib for group3, Alcoholic extract and Sorafenib for group4, CCl4 for group5, Alcoholic extract and CCl4 for group6, Sorafenib and CCl4 for group7, and Alcoholic extract, Sorafenib, and CCl4 group8. The biochemical data were analyzed with SPSS software and represented as means ± SD before being submitted to one-way analysis of variance (ANOVA) and post-hoc Tukey tests. PNO2 and GSH levels fell significantly (p <0.05) in the CCl4 group compared to the control group, according to the results obtained for the specified biochemical parameters. When comparing the CCl4 group to the other groups, the levels of AFP and MDA rose considerably (p <0.05). The fruit extract of Pistacia khinjuk exhibits hypocholesterolaemic qualities, a hepatoprotective impact, enhances GSH, PNO2, and decreases MDA, according to this study. hepatic disorders may all benefit greatly from this treatment.

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Journal ID : BNIHS-19-03-2022-10207
Title : Evaluation of Aflatoxin B1 detoxification by using green Ag nanoparticles synthesis from some plant extracts
Abstract :

A four medicinal leaves plants were gainted from local market and the alcohol extraction were doing using 75% methanol. Green silver nanoparticles synthesis from foure medicinal plant extracts. Scanning electron microscopy (SEM), UV visible spectroscopy, Fourier Transform Infrared Spectroscopy(FTIR), and Energy Dispersive Spectroscopy (EDS). The results showed nanoparticles, smooth spherical nanoparticles ranging in size from 29.03 to 88.00 nm. The standard concentration of aflatoxin B1 is (29) ppb, according to the findings of a study on the effectiveness of green nanoparticles against aflatoxin B1 using high-performance liquid chromatography technology to assess its concentration. The extracts were examined by HPLC, and found that the concentration of aflatoxin B1 is The present in the alcoholic extracts. (Thymus vulgaris: 0.065ppb, Rosmarinus officinalis:0.580ppb, Mentha spicata:0.377 ppb, and Eucalyptus camaldulensis:1.067ppb). But Ag nanoparticles alcohol extracts (T. vulgaris:rare, R. officinalis:0.065ppb, M. spicata:1.083 ppb, and E. camaldulensis:0.615ppb). The remove rate in green Ag nanoparticles of alcohol extract showed aflatoxin B1 detoxification ratios by using rosemary: 99.67 % eucalyptus: 96.92 %, mint: 94.58%, and thyme: 92.07%. However, that alcoholic extracts of thyme: 99.67%, mint: 98.15%, rosemary: 98.15% and eucalyptus: 96.49%. From the results appeared the superiority of the thyme plant detoxification over the rest of the plant extracts while rosemary Ag nanoparticles have higher in aflatoxin B1 detoxification activity than other nanoparticles. The mint plant showed less effectiveness against aflatoxin B1.

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Journal ID : BNIHS-18-03-2022-10206
Title : The impact of angiotensin II type 1 receptor (ATR1) gene polymorphism (A1166C) on cardiovascular and renal complications in Iraqi hypertensive patients treated with candesartan
Abstract :

Angiotensin II is the most vasoactive peptide and a powerful blood vessels constrictor of renin-angiotensin-aldosterone system (RAAS). It affects arterial musculature, increases peripheral resistance, and raises blood pressure (BP). The majority of angiotensin II's effects are mediated by the angiotensin II type 1 receptor (ATR1). To investigate the impact of A1166C single nucleotide polymorphism (SNP) of ATR1 gene on cardiovascular and renal complications in Iraqi hypertensive patients treated with candesartan. Ninety-two patients with essential hypertension taking 8 mg/day candesartan from not less than three months were recruited for the investigation from Imam Hussein Teaching Hospital and from private clinic in Karbala province, Iraq. Blood pressure, heart rate and echocardiography reports were recorded. The analysis of biochemical parameters such as serum creatinine, urea, angiotensinogen, angiotensin II and some electrolytes levels were done. The tetra-primer amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) was used for genotyping of the A1166C SNP. The distribution of A1166C SNP in the hypertensive patients was 54.35% AA, 40.22% AC and 5.43% CC. There was no association between A1166C and echocardiogram, biochemical or BP parameters (P>0.05) except heart rate; patients with AA genotype have higher heart rate (88.32±10.64, P=0.005) than patients with AC and CC genotype. The CC genotype of A1166C is the less frequent than other two genotypes of this SNP in Iraqi hypertensive patients. The A1166C SNP has neither an effect on hypertension complication nor on the patients’ response to candesartan in Iraqi hypertensive patients.

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Journal ID : BNIHS-18-03-2022-10205
Title : Expression of a cell cycle regulatory protein (P73) correlated with IL-10 levels in HPV-infected gastric cancer patients
Abstract :

Gastric cancer is a multifactorial disease in which numerous factors, microbial infections have been shown to contribute to gastric tumorigenesis. HPV is among the well-known causes of cancer infectious agents. HPV is divided into two categories: low-risk HPVs that cause anogenital and cutaneous warts, and high-risk HPVs that cause oropharyngeal cancers as well as anogenital cancers. P73 is a transcription factor that belongs to the p53 gene family, p73 gene produces two types of proteins: full-length isoforms (TAp73), which act as transcription factors, and N-terminal truncated variants (∆Np73), which lack the TAD, resulting in transcriptionally inactive isoforms that behave as oncogenes in a dominant-negative way. IL-10 is an important regulatory cytokine with anti-inflammatory properties. IL-10 is one of the several important cytokines involved in cancer development and sustenance. The study aims to explore a possible etiological association between high oncogenic-risk HPV genotype (16, 18, 31, 33) DNA and GC, also to evaluate the immunohistochemical expression of p73 and IL-10 proteins in GC tissues. This study involved 84 selected formalin-fixed, paraffin-embedded tissue blocks samples, the collected samples were divided into the following study groups: 54 blocks of GC mass tissues and 30 non-malignant gastric tissues used as a control group for this study. CISH was used to detect HPV DNA16/18 and DNA31/33 in gastric tissues. Regarding the mass GC group, the total percentage of positive HPV16/18 and 31/33 was 44.4%, whereas in the non-malignant group HPV16/18 and 31/33 DNA constituted 80.0%. Also, the total percentage of positive p73-IHC detection was 63%, whereas in the non-malignant group p73 constituted 60%. IL-10 was 74.1% in malignant gastric tissues and 40.0% in non-malignant gastric tissues. These data support previous studies suggesting a role for HPV infection in GC and also provide evidence for an association between HPV infection and p73 with IL-10 proteins expression in GC.

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Journal ID : BNIHS-17-03-2022-10204
Title : Studying the protective and therapeutic role of Rutin on the histological structure of the liver and some physiological parameters in Rats treated with ciprofloxacin
Abstract :

Antibiotics have great benefits, however, not free from side effects. Its side effects, especially those related to liver damage and increased levels of liver enzymes, have prompted the world to search for a traditional medicinal plant such as Rutin to find preventive and curative agents against multi-organ dysfunction (unwanted effects) of Ciprofloxacin. The aim of this study is to evaluate the preventive and therapeutic effect of Rutin in inducing liver injury in animals by Ciprofloxacin. The study included Six groups, each group contains 5 male albino rats and the dose was taken orally daily, The control group were given normal drinking water for 14 days, While The first treatment group (T1) were given Rutin concentration of 50mg/kg of body weight for 14 days, and The second treatment group (T2) were given Ciprofloxacin antibiotic, and it’s concentration equal 14mg/kg of body weight for 14 days, The third treatment group (T3) were given Rutin concentration of 50mg/kg of body weight for 14days and then Ciprofloxacin oral given with a dosage of 14mg/kg of body weight for 14 days, The fourth treatment group (T4) were given Ciprofloxacin antibiotic concentration of 14mg/kg of body weight and then Rutin oral given with a dosage of 50mg/kg of body weight in combination for 14 days and The fifth treatment group (T5) was given Ciprofloxacin antibiotic concentration of 14mg/kg of body weight for 14 days and then Rutin with concentration equal 50mg/kg of body weight for 14 days. histopathological changes occurred in the liver of the animals of the group (T2) represented with Loss of the normal hexagonal arrangement of hepatocytes, cellular dissociation, congestion in a central vein, fatty necrosis, and lymphoid infiltration compared with other groups. Rutin treatment at various doses has hepatoprotective effect by maintaining the functional integrity by improving the cellular antioxidant status, reducing oxidative stress, and preserving the integrity of the histomorphological structure of the liver. It was concluded that Rutin at 50mg/kg has an important hepatoprotective effect against injury induced by Ciprofloxacin in the liver.

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